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Planta Med 1997; 63(1): 66-69
DOI: 10.1055/s-2006-957607
Papers
Phytochemical Analysis
© Georg Thieme Verlag Stuttgart · New York

A High-Performance Liquid Chromatography Analysis of Plasma Artemisinin using a Glassy Carbon Electrode for Reductive Electrochemical Detection

Kit Lam Chan, Kah Hay Yuen, Sunil Jinadasa, Kok Khiang Peh, Weng Tuck Toh
  • School of Pharmaceutical Science, University of Sicence Malaysia, 11800 Penang, Malaysia
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Publication History

1996

1996

Publication Date:
04 January 2007 (online)

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Abstract

A high-performance liquid chromatography assay equipped with a glassy carbon electrode for electrochemical detection (HPLC-ECD) was developed at reductive mode for the analysis of artemisinin, the antimalarial drug from Artemisia annua (Asteraceae) in human plasma. This method was selective, sensitive, and produced satisfactory recovery, precision, and accuracy. Analysis of plasma samples from 8 male volunteers given 10 mg kg-1 of artemisinin orally as an aqueous suspension showed a mean peak plasma concentration (Cmax) of 580.89 ng ml-1 ± 88.64 SD at 2.5 h ± 0.5 SD after dosing, and the mean area under the plasma concentration-time curve (AUC0-∞) was 2227.57 ng h ml-1 ± 677.22 SD. In addition, the elimination rate constant (Ke), elimination half-life (t1/2), and apparent volume of distribution (Vd) were calculated to be 0.2971 h- ± 0.0644 SD, 2.42 h ± 0.46 SD, and 16.26l kg-1 ± 3.44 SD, respectively.

Key words

Artemisia annua - Asteraceae - artemisinin analysis - HPLC-ECD - pharmacokinetics

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