Experimental Treatment of Cutaneous Leishmaniasis with Argentilactone Isolated from Annona haematantha

Abstract

From the hexanic extract of roots of Annona haematantha an α,β-unsaturated δ-lactone was isolated and identified as argentilactone. This compound exhibited in vitro activity against various strains of Leishmania ssp. at 10 µg/ml. BALB/c mice infected with Leishmania amazonensis were treated four weeks after infection with argentilactone by oral or subcutaneous routes for 14 days at 25 mg/kg daily. The reference drug, N-methylglucamine antimonate, was administered by subcutaneous injections at l00 mg/kg for 14 days. In these conditions, argentilactone showed the same efficacy as the reference drug, reducing by 96% the parasite loads in the lesion and by 50% the parasite burden in spleen.

key words

Annona haematantha - Annonaceae - argentilactone - BALB/c mice - Leishmania amazonensis