Agrobacterium rhizogenes-Mediated Transformation of Artemisia annua: Production of Transgenic Plants

S. Banerjee; M. Zehra; M. M. Gupta; S. Kumar

doi:10.1055/s-2006-957737

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Planta Med 1997; 63(5): 467-469
DOI: 10.1055/s-2006-957737

Letters

Agrobacterium rhizogenes-Mediated Transformation of Artemisia annua: Production of Transgenic Plants

S. Banerjee¹ , M. Zehra¹ , M. M. Gupta² , S. Kumar¹

¹Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), P. O. CIMAP, Lucknow-226 015, India
²Analytical Chemistry, Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow-226 015, India

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Publication History

1996

1997

Publication Date:
04 January 2007 (online)

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Abstract

Transgenic hairy roots were induced in the leaves of Artemisia annua by treatment with the LBA 9402 strain of Agrobacterium rhizogenes. The axenic hairy root cultures were found to produce the sesquiterpenes artemisinic acid and arteannuin B. The hairy root cultures were observed to spontaneously regenerate into plantlets on solid hormone-free MS medium. The regenerated plants had phenotypic characteristics typical to the transformed plants. Among the plants of the age of one month in culture, the transgenic plant was bigger (2.643 g/plant) than the normal (0.856 g/plant). Both these kinds of in vitro plants carried sesquiterpenes-artemisinic acid and arteannuin B.