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Planta Med 1997; 63(6): 499-502
DOI: 10.1055/s-2006-957750
Papers
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Differentiation of Human Gastric Adenocarcinoma Cell Line MGc80-3 Induced by Verbascoside

Li Ji1 , Zheng Yun2 , Zhou Hong2 , Su Baoning3 , Zheng Rongliang1
  • 1Department of Biology, Lanzhou University, Lanzhou 730000, People's Republic of China
  • 2Cancer Research Centre, Xiamen University, Xiamen 361005, People's Republic of China
  • 3State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China
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Publication History

1996

1997

Publication Date:
04 January 2007 (online)

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Abstract

Verbascoside is a natural antioxidant extracted from Pedicularis striata Pall (Jueyehesen). After being treated with 20 µmol/l verbascoside, the growth curve and mitotic index of human gastric adenocarcinoma MGc80-3 cells decreased remarkably, cell doubling time was delayed, the cellular growth inhibitory rate amounted to 53.2%, cell surface charge assayed by cell electrophoresis obviously changed, the electrophoresis rate dropped from 3.51 µm/s/v/cm to 2.74, i.e., the percent of retardation reached 28.4%. There was a 75% decrease of the tumorigenicity for the treated cells compared with the untreated cells inoculated subcutaneously in BALB/C nude mice. Scanning electron microscopy revealed that the microvilli on the surface of treated cells had been reduced obviously. It confirmed that verbascoside, similar to DMSO, could reverse MGc80-3 cells' malignant phenotypic characteristics and induced redifferentiation of MGc80-3 cells.

Key words

Verbascoside - Pedicularis striata - Scrophulariaceae - antioxidant - human gastric adenocarcinoma MGc80-3 - redifferentiation