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Planta Med 1996; 62(2): 169-172
DOI: 10.1055/s-2006-957844
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© Georg Thieme Verlag Stuttgart · New York

Rapid Analysis of Small Samples Containing Forskolin Using Monoclonal Antibodies

H. Yanighara1 , R. Sakata1 , Y. Shoyama1 , H. Murakami2
  • 1Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan
  • 2Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812, Japan
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Publication History

1994

1995

Publication Date:
04 January 2007 (online)

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Abstract

The effective range of the competitive ELISA test for detection of forskolin content in clonally propagated plant organs of Coleus forskohlii using monoclonal antibodies extends from 5 ng to 5 µg. A correlation between the forskolin accumulation and the growth rate was investigated using the clonally propagated shoots. An increase of forskolin content was noted, beginning at week 6. Flowers, rachises, leaves, stems, tuberous roots, and roots were analyzed. Tuberous roots and the stem base contained higher amounts of forskolin than other organs. The forskolin content in the stem decreased gradually towards the top of the shoot.

Key words

Forskolin - Coleus forskohlii - Lamiaceae - clonal propagation - enzyme-linked immunosorbent assay - high-performance liquid chromatography - tissue distribution