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Planta Med 1996; 62(3): 263-266
DOI: 10.1055/s-2006-957874
Paper

© Georg Thieme Verlag Stuttgart · New York

Determination of Wogonin in Rat Plasma by Liquid Chromatography and its Pharmacokinetic Application

T. H. Tsai1 , 2 , C. J. Chou1 , T. R. Tsai3 , C. F. Chen1 , 4
  • 1Department of Pharmacology, National Research Institute of Chinese Medicine, Shih-Pai, Taipei 11221, Taiwan
  • 2Institute of Traditional Medicine, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan
  • 3Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College, Kaohsiung 807, Taiwan
  • 4Department of Pharmacology, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan
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Publication History

1995

1995

Publication Date:
04 January 2007 (online)

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Abstract

A high-performance liquid chromatographic method has been developed for the determination of wogonin in the rat plasma. After addition of an internal standard (clomipramine), plasma was deproteinized by acetonitrile and centrifuged for sample clean-up. The supernatant was separated by a reversed-phase column chromatography, identified by a photodiode-array detector, and quantified by ultraviolet detection at a wavelength of 275 nm. Acetonitrile-water-diethylamine (50:50:0.1, v/v/v, pH 3.0 adjusted by orthophosphoric acid) was used as the mobile phase. The method was applied to the pharmacokinetic study of wogonin in rats after a dose of 5 mg/kg by intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration time profile.

Key words

Wogonin - 5,7-dihydroxy-8-methoxy-2-phenyl-4H-1-benzopyran-4-one - pharmacokinetics - HPLC - photodiode-array detection