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Planta Med 1996; 62(3): 281-283
DOI: 10.1055/s-2006-957881
Letter

© Georg Thieme Verlag Stuttgart · New York

Clonal Propagation of Cnidium officinale by Shoot Tip Culture

Bijaya Pant, Hiroshi Kohda, Akira Namera
  • Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734, Japan
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Publication History

1995

1995

Publication Date:
04 January 2007 (online)

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Abstract

Multiple shoots were induced from the apical domes of shoot tips of Cnidium officinale Makino (Apiaceae) by culturing them on Murashige and Skoog (MS) 1 static media solidified with 0.2% gelrite and supplemented with 6-benzylaminopurine (BAP) 10-6M. An average of 5.3 shoots per segment were obtained within 6 weeks and this ability did not decline even after two years of subculture. Subsequent transfer of these regenerated shoots on MS media supplemented with α-naphthaleneacetic acid (NAA) 10-7M and BAP 10-7M resulted in root formation. Rooted plantlets were able to grow in soil after a short period of acclimatization.

Cytological observation in root tip cells of cultivated, as well as in vitro propagated plantlets revealed that in both cases the cells had 2n = 22 chromosomes indicating the homogeneity of the clonally propagated plants.