A Water-Soluble Antitumor Glycoprotein from Chlorella vulgaris

Kiyoshi Noda; Naohito Ohno; Kuniaki Tanaka; Norikiyo Kamiya; Masao Okuda; Toshiro Yadomae; Kikuo Nomoto; Yukihiro Shoyama

doi:10.1055/s-2006-957931

Planta Med 1996; 62(5): 423-426
DOI: 10.1055/s-2006-957931

Paper

A Water-Soluble Antitumor Glycoprotein from Chlorella vulgaris

Kiyoshi Noda¹ , Naohito Ohno² , Kuniaki Tanaka³ , Norikiyo Kamiya³ , Masao Okuda³ , Toshiro Yadomae² , Kikuo Nomoto⁴ , Yukihiro Shoyama¹

¹Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan
²Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan
³Research Laboratories, Chlorella Industries Co. Ltd., 1343 Hisatomi, Chikugo City, Fukuoka 833, Japan
⁴Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan

Abstract

als PDF herunterladen

Abstract

An active substance with antitumor activity (ARS2) was purified from the culture media of Chlorella vulgaris and found to be a glycoprotein with a molecular weight of 63,100 amu, as determined by matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry. ARS2 contains 66.9% carbohydrate, mainly D-galactose, and 35.2% protein. The carbohydrate moiety has a β-1,6-D-galactopyranose backbone, as determined by methylation analysis and ¹³C-NMR. Apparently, the protein moiety, whose 15 amino acid sequence at the NH₂-terminus, we determined as DVGEAFPTWDALVA, is necessary for the antitumor activity, as assessed by hydrazinolysis, periodate oxidation, and proteolysis.

Key words

Chlorella vulgaris - Chlorococcales - galactose-rich glycoprotein - methylation analysis - ¹³C-NMR - antitumor activity

PDF (338 kb)