Influence of the Galactoside-Specific Lectin from Viscum album and its Subunits on Cell Aggregation and Selected Intracellular Parameters of Rat Thymocytes

Abstract

Galactose-specific lectin from Viscum album (VAA) was shown to induce the aggregation of rat thymocytes, to increase the intracellular Ca²⁺- availability, and to enhance the menadione-dependent release of H₂O₂ from cells in a carbohydrate- and dose-dependent manner. This activity was mediated by the carbohydrate-binding B-subunit, whereas the toxic A-subunit failed to display aggregating activity or to affect the intracellular Ca²⁺. However, incubation of cells in its presence decreased the release of H₂O₂. These data extend the knowledge of the immunomodulatory potency of VAA, especially its carbohydrate-binding B chain.

Lectin - Viscum album - Viscaceae - rat thymocytes - cell aggregation - intracellular calcium - hydrogen peroxide