The performance of four chiral liquid chromatographic columns (Chiralcel® OA, OJ, OC, OD) was studied with respect to the enantioseparation of flavanones and some derivatives. The effect of mobile phase composition on the retention time and stereo-selectivity was studied. A good enantioseparation (α up to 1.45) was achieved for most of the racemates. Further, the elution order of the enantiomers from the chiral stationary phases has been determined by using a circular dichroism based detection system.
Column liquid chromatography - chiral stationary phases - flavanones - enantiomeric separation - elution order - circular dichroism detection system
