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Planta Med 1994; 60(5): 441-444
DOI: 10.1055/s-2006-959528
Paper

© Georg Thieme Verlag Stuttgart · New York

Biotransformation of the Naturally Occurring Lignan (-)-Arctigenin in Mammalian Cell Lines Genetically Engineered for Expression of Single Cytochrome P450 Isoforms

R. Kasper1 , D. Ganßer2 , J. Doehmer3
  • 1Institut für Pharmazeutische Biologie, Freie Universität, Königin-Luise-Str. 2 + 4, D-14195 Berlin-Dahlem, Federal Republic of Germany
  • 2Lehrstuhl Organische Chemie I, Universität Bayreuth, D-95440 Bayreuth, Federal Republic of Germany
  • 3Institut für Toxikologie und Umwelthygiene, Technische Universität, D-80636 München, Federal Republic of Germany
Further Information

Publication History

1993

1994

Publication Date:
04 January 2007 (online)

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Abstract

V79 Chinese hamster cells genetically engineered for stable expression of single forms of rat cytochromes P450 1A1, P450 1A2, and P450 2B1, and human P450 1A2 were used to analyse the metabolism of (-)-arctigenin. Concentrations of metabolites formed in the medium were determined by TLC, HPLC, GC, and GC-MS. Only in the cell line expressing rat P450 2B1 was (-)-artigenin metabolized to a significant extent. The lignan was converted by O-demethylation to (-)-3′-O-demethylarctigenin.

Key words

(-)-Arctigenin - biotransformation - V79 - cytochrome P450 - lignan