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Planta Med 1993; 59(3): 221-228
DOI: 10.1055/s-2006-959656
Paper

© Georg Thieme Verlag Stuttgart · New York

Cross-Reactivity Between Pollen Extracts from Six Artemisia Species

J. Brandys1 , A. Grimsøen1 , B. M. Nilsen1 , B. Smestad Paulsen1 , 3 , H. S. Park2 , C. S. Hong2
  • 1Institute of Pharmacy, Department of Pharmacognosy, P.O.Box 1068 Blindern, 0316 Oslo 3, Norway
  • 2Department of Internal Medicine, Yonsei University College of Medicine, C.P.O. BOX 8044, Seoul, Korea
  • 3Address for correspondence
Further Information

Publication History

1992

1993

Publication Date:
04 January 2007 (online)

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Abstract

Pollen extracts of six different Artemisia species, A. vulgaris, A. scoparia, A. princeps, A. tridentata, A. annua, and A. campestris were compared using SDS-PAGE, IEF, immunoblotting, and immunoelectrophoretic methods. The band patterns obtained after SDS-PAGE and IEF showed a large degree of similarity between the extracts. Immunoblotting of these gels using a pool of sera from patients allergic to A. vulgaris gave essentially the same IgE-binding band pattern with all the extracts, demonstrating an extensive degree of cross-reactivity between A. vulgaris and the other Artemisia species. FRIE using a polyspecific antiserum against A. vulgaris showed that all the extracts contained several antigens that were immunologically identical to antigens in A. vulgaris extract. Antigens showing immunological identity to the important A. vulgaris allergens Ag 12 and Art v II were present in all the extracts. The cross-reactivity between A. vulgaris and A. princeps was further verified by screening of ten Korean and nine Norwegian individual patient sera against extracts of both species in SDS-PAGE or IEF immunoblotting. Both groups of patients had essentially the same pattern of reactivity towards both pollen extracts.

Key words

Artemisia spp. - pollen - allergens - cross-reactivity.