Tropane Alkaloid Production in Transformed Root Cultures of Brugmansia candida

A. M. Giulietti; A. J. Parr; M. J. C. Rhodes

doi:10.1055/s-2006-959725

Planta Medica, Table of Contents

Planta Med 1993; 59(5): 428-431
DOI: 10.1055/s-2006-959725

Paper

Tropane Alkaloid Production in Transformed Root Cultures of Brugmansia candida

A. M. Giulietti¹ ^, ² , A. J. Parr¹ , M. J. C. Rhodes¹ ^, ³

¹Plant Biotechnology Group, Department of Genetics and Microbiology, AFRC Institute of Food Research - Norwich Laboratory, Colney Lane, Norwich NR4 7UA, U.K.
²Permanent address: Biotecnologia y Microbiologia Industrial, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina

Abstract

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Abstract

Transformed root cultures of Brugmansia candida were established by infection with Agrobacterium rhizogenes LBA 9402. Several clones with different growth index and tropane alkaloid pattern and content were obtained and two were examined in depth. The alkaloid content and pattern changed during the time course. At 21 days of culture clone 1 revealed an alkaloid spectrum dominated by 3α-acetoxytropane (about 50% of the total alkaloid) and hyoscyamine (about 25%), with a ratio of hyoscyamine to scopolamine of 11.2. In clone 40 this ratio was 1.5 and the content of 3α-acetoxytropane was low (2%). The maximum concentrations of hyoscyamine were obtained at 3 weeks of culture, and were 700 and 500 µg/g FW in clone 1 and 40, respectively.

Key words

Brugmansia candida - transformed roots - tropane alkaloids - hairy roots

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