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Planta Med 1991; 57(5): 425-427
DOI: 10.1055/s-2006-960140
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© Georg Thieme Verlag Stuttgart · New York

Incorporation of [1-14 C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium

S. William Zito1 , Vimal Srivastava1 , 2 , Esther Adebayo-Olojo1
  • 1Department of Pharmaceutical Sciences, St. John's University, College of Pharmacy and Allied Health Professions, Jamaica, New York 11439, U.S.A.
  • 2Bethesda Research Laboratories, Gaithersburg, MD 20877, U.S.A.
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Publication History

1990

Publication Date:
05 January 2007 (online)

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Abstract

Callus culture of Chrysanthemum cinerariaefolium (Trev.) Bocc. (Asteraceae), initiated with axillary buds from the plant, have been shown to produce chrysanthemyl alcohol and chrysanthemic acid. Incubation of a cell-free homogenate from the callus with [1-14 C]-isopentenyl pyrophosphate demonstrated incorporation of radioactivity into chrysanthemyl alcohol and chrysanthemic acid as well as products from isopentenyl pyrophosphate isomerase (EC 5.3.3.2; isopentenyl diphosphate Δ3-Δ2-isomerase) and prenyltransferase activities (EC 2.5.1.11; allylic-terpene-diphosphate:isopentenyl diphosphate terpenoid-allyltransferase).

Key words

Pyrethrum - Chrysanthemum cinerariaefolium - tissue culture - chrysanthemyl alcohol - chrysanthemic acid - pyrethrins - monoterpene biosynthesis - isopentenyl pyrophosphate isomerase - prenyltransferase