Incorporation of [1-14C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium

S. William Zito; Vimal Srivastava; Esther Adebayo-Olojo

doi:10.1055/s-2006-960140

Planta Medica, Inhaltsverzeichnis

Planta Med 1991; 57(5): 425-427
DOI: 10.1055/s-2006-960140

Papers

Incorporation of [1-¹⁴ C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium

S. William Zito¹ , Vimal Srivastava¹ ^, ² , Esther Adebayo-Olojo¹

¹Department of Pharmaceutical Sciences, St. John's University, College of Pharmacy and Allied Health Professions, Jamaica, New York 11439, U.S.A.
²Bethesda Research Laboratories, Gaithersburg, MD 20877, U.S.A.

Abstract

als PDF herunterladen

Abstract

Callus culture of Chrysanthemum cinerariaefolium (Trev.) Bocc. (Asteraceae), initiated with axillary buds from the plant, have been shown to produce chrysanthemyl alcohol and chrysanthemic acid. Incubation of a cell-free homogenate from the callus with [1-¹⁴ C]-isopentenyl pyrophosphate demonstrated incorporation of radioactivity into chrysanthemyl alcohol and chrysanthemic acid as well as products from isopentenyl pyrophosphate isomerase (EC 5.3.3.2; isopentenyl diphosphate Δ³-Δ²-isomerase) and prenyltransferase activities (EC 2.5.1.11; allylic-terpene-diphosphate:isopentenyl diphosphate terpenoid-allyltransferase).

Key words

Pyrethrum - Chrysanthemum cinerariaefolium - tissue culture - chrysanthemyl alcohol - chrysanthemic acid - pyrethrins - monoterpene biosynthesis - isopentenyl pyrophosphate isomerase - prenyltransferase

PDF (247 kb)

Incorporation of [1-14 C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium

Incorporation of [1-¹⁴ C]-Isopentenyl Pyrophosphate into Monoterpenes by a Cell-Free Homogenate Prepared from Callus Cultures of Chrysanthemum cinerariaefolium