Identification of Melissa officinalis Subspecies by DNA Fingerprinting

Hans-Thomas Wolf; Thomas van den Berg; Franz-Christian Czygan; Armin Mosandl; Thomas Winckler; Ilse Zündorf; Theodor Dingermann

doi:10.1055/s-2006-960447

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Planta Med 1999; 65(1): 83-85
DOI: 10.1055/s-2006-960447

Letter

Identification of Melissa officinalis Subspecies by DNA Fingerprinting

Hans-Thomas Wolf¹ , Thomas van den Berg² , Franz-Christian Czygan² , Armin Mosandl³ , Thomas Winckler¹ , Ilse Zündorf¹ , Theodor Dingermann¹

¹Institut für Pharmazeutische Biologie, Universität Frankfurt, Frankfurt/M., Germany
²Julius-von-Sachs-Institut für Biowissenschaften mit Botanischem Garten, Würzburg, Germany
³Institut für Lebensmittelchemie, Universität Frankfurt, Frankfurt/M., Germany

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1998

1998

Publication Date:
04 January 2007 (online)

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Abstract

The random amplified polymorphic DNA analysis (RAPD) is a method to study genetic variability within and between populations and species on the basis of the amplification of anonymous fragments from genomic DNA templates by means of polymerase chain reaction (PCR). We applied RAPD analysis in order to distinguish medicinal plant subspecies at the level of their genomes. In this study we investigated various samples of two Melissa subspecies and showed that RAPD analysis is a fast and reliable method to distinguish subspecies on the pharmaceutical market that have been previously classified according to the distribution pattern of compounds present in the lemon balm oil.