Abstract
From the leaves of Cupressocyparis leylandii (Cupres-saceae) cupressuflavone, 4′-O-methylcupressuflavone, amentoflavone, 7-O-methylamentoflavone, 4‴-O-methylamentoflavone and hinokiflavone were isolated. 1H- and 13C-NMR data for 4′-O-methylcupressuflavone are given for the first time. The biflavones from cultivar varieties of C. leylandii (Naylor's Blue, Castlewellan Gold) were chromatographically (HPLC) compared. The antifungal activity of cupressuflavone and 4′-O-methylcupressuflavone against Alternoria alternato, Cladosporium oxysporum, Fusarium culmorum and F. avenaceum was assayed.