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Planta Med 1999; 65(7): 656-658
DOI: 10.1055/s-2006-960840
Letter

© Georg Thieme Verlag Stuttgart · New York

In Vitro Inducible Nitric Oxide Synthesis Inhibitory Active Constituents from Fraxinus rhynchophylla

Na-Young Kim1 , Hyun-Ock Pae2 , Yong-Suk Ko1 , Ji-Chang Yoo2 , Byung-Min Choi2 , Chang-Duk Jun2 , Hun-Taeg Chung2 , M. Inagaki3 , R. Higuchi3 , Youn-Chul Kim1
  • 1College of Pharmacy, Wonkwang University, Iksan, Korea
  • 2Wonkwang University School of Medicine and Medicinal Resources Research Center, Iksan, Korea
  • 3Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
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Publication History

1998

1999

Publication Date:
04 January 2007 (online)

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Abstract

Bioassay-guided fractionation of an H2O extract of the barks of Fraxinus rhynchophylla has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, ferulaldehyde (1) and scopoletin (3) together with a coumarin, fraxidin (2). Compounds 1 and 3 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner by murine macrophage-like RAW 264.7 cells stimulated with interferon-γ (IFN-γ) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was reflected in the decreased amount of iNOS protein, as determined by Western blotting.