Download PDF
Planta Med 1992; 58(4): 314-316
DOI: 10.1055/s-2006-961474
Papers

© Georg Thieme Verlag Stuttgart · New York

Effects of Genistein, An Isoflavone Isolated from Genista tridentata, on Isolated Guinea-Pig Ileum and Guinea-Pig Ileal Myenteric Plexus

M. D. Herrera1 , E. Marhuenda1 , A. Gibson2
  • 1Departamento de Farmacia y Tecnología Farmaceútica. Facultad de Farmacia. Universidad de Sevilla, Sevilla, Spain
  • 2King's College London, Pharmacology Department, University of London, London, U.K.
Further Information

Publication History

1991

Publication Date:
05 January 2007 (online)

Also available at
  PDF Download  Permissions and Reprints

Abstract

The inhibitory action of the major constituent of Genista tridentata L. (Papilionaceae), 4′,5,7-trihydroxyisoflavone (genistein), on contractions induced by agonists and electrical field stimulation of smooth muscle was analysed. Genistein inhibited twitches evoked by electrical-stimulation of strips of guinea-pig ileum with an IC50 value of 34 µM. Genistein (34 µM) inhibited contractions of the guinea-pig ileum by several agonists in a non-selective, antispasmodic action and had no effect on inhibition of 3H-ACh release from ileal myenteric plexus. Genistein (34 µM) produces an increase in cAMP levels of guinea-pig ileum which resulted in a smooth muscle relaxation which leads us to think that there must be a blockade of its phosphodiesterase.

Key words

Genista tridentata - Papilionaceae - genistein - antispasmodic activity