Effects of Chinese Drugs “Xiebai” and “Dasuan” on Human Platelet Aggregation¹ (Allium bakeri, A. Sativum)

Abstract

Adenosine (1), guanosine (2), and tryptophan (3), as well as (β-sitosterol (β-D-glucoside (4) were isolated from the n-butanol-soluble fraction of Xiebai, the tuber of Allium bakeri Reg., which was recognized to have an anti-platelet aggregation effect. Moreover, 1 and 2 were also isolated from the n-butanol-soluble fraction of Dasuan, the tuber of A. sativum L. Compound 1 showed a significant inhibitory activity against both the primary and secondary wave aggregation of human platelet induced by 2 µM ADP, whereas compounds 2-4 showed no or very low inhibitory effects. The structure-activity relationships on human platelet aggregation with regard to 1, 2 and their derivatives, adenosine 2′-monophosphate (5), adenosine 5′-monophosphate (6), adenosine triphosphate (7), guanosine 3′-monophosphate (8), and guanosine 5′-monophosphate (9), and guanylyl(3′→5′)adenosine (10) are discussed. Compounds 5-7 and 10 also inhibited the primary and secondary wave aggregation with a dose-dependent response.