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Planta Med 1988; 54(1): 63-65
DOI: 10.1055/s-2006-962340
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© Georg Thieme Verlag Stuttgart · New York

Radioimmunoassay for Rapid Estimation of Vitamin D Derivatives in Calcinogenic Plants

Martin Weissenberg1 , Arieh Maoz2 , Arieh Levy3 , Robert H. Wasserman4
  • 1Department of Chemistry of Natural Products and Pesticides, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel.
  • 2Institute of Plant Protection, Agricultural Resarch Organization, The Volcani Center, Bet Dagan 50 250, Israel.
  • 3Department of Medicinal and Spice Crops, Agricultural Research Organization, The Volcani Center, Bet Dagan 50 250, Israel.
  • 4New York State College of Veterinary Medicine, Cornell University, Ithaca, New York, U.S.A.
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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

A radioimmunoassay is described for the rapid measurement of vitamin D derivatives in calcinogenic plants, using a commercially available antibody. A high specificity of the antibody for the 25-hydroxy group was detected in a series of synthetic vitamin D derivatives, and its steric requirements are discussed. The advantages and utility of this assay for large screening programs, plant selection and breeding, tissue culture studies and purification work, are evaluated.