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Planta Med 1988; 54(1): 66-68
DOI: 10.1055/s-2006-962341
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© Georg Thieme Verlag Stuttgart · New York

Quantitative Bestimmung von Secoiridoidglucosiden aus den Früchten von Ligustrum vulgare mit HPLC

Quantitative Determination of Seco-Iridoid Glucosides from the Fruits of Ligustrum vulgare by HPLCMichael Willems
  • Institut für Pharmazeutische Biologie, Johann Wolfgang-Goethe-Universität Frankfurt/Main, D-6000 Frankfurt/Main, Bundesrepublik Deutschland.
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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

A HPLC method for separation and quantitative determination of secoiridoid glucosides in fruit extracts from Ligustrum vulgare L. is described. A system using a reversed phase column and an ethanol-water gradient elution, was suitable for separation of Ligstroside, Oleuropein, Nüzhenide and LF (3) 8. The secoiridoid glucosides were detected at 235 nm. The detection limits were within 2 and 10 µg/ml. Ripe fruits yield about 8.85% of secoiridoid glucosides (dried weight).