Phorbol Diester Constituents of Croton Oil: Separation by Two-Dimensional TLC and Rapid Purification Utilizing Reversed-Phase Overpressure Layer Chromatography (RP-OPLC)

Clemens A. J. Erdelmeier; Patricia A. S. Van Leeuwen; A. Douglas Kinghorn

doi:10.1055/s-2006-962343

Planta Medica, Table of Contents

Planta Med 1988; 54(1): 71-75
DOI: 10.1055/s-2006-962343

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Phorbol Diester Constituents of Croton Oil: Separation by Two-Dimensional TLC and Rapid Purification Utilizing Reversed-Phase Overpressure Layer Chromatography (RP-OPLC)

Clemens A. J. Erdelmeier¹ , Patricia A. S. Van Leeuwen² , A. Douglas Kinghorn¹

¹Program for Collaborative Research in the Pharmaceutical Sciences, and Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, U.S.A.
²Clinical Pharmacokinetics Laboratory, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, U.S.A.

Abstract

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Abstract

Fourteen known phorbol diester constituents of Croton tiglium L. seed oil (croton oil) have been separated by a two-dimensional thin-layer chromatographic (2D-TLC) technique using different sorbent layers on the same plate. After a preliminary purification step using low-pressure liquid chromatography, reversed-phase overpressure liquid chromatography (RP-OPLC) has been shown to be potentially useful for the rapid preparative purification of these toxins. The identity of the major croton oil phorbol diesters was established by co-chromatography with authentic compounds and/or by off-line chemical ionization mass spectrometry. The standard tumor-promoting agent, 12-O-tetradecanoylphorbol-13-acetate, was obtained free from impurities as a result of the application of both the 2D-TLC and liquid chromatography-RP-OPLC procedures.

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