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Planta Med 1988; 54(1): 75-79
DOI: 10.1055/s-2006-962344
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© Georg Thieme Verlag Stuttgart · New York

Multiplication of Curcuma Species by Tissue Culture

Kazumi Yasuda, Tadashi Tsuda, Harumi Shimizu, Aiko Sugaya
  • Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyakidai, Sakado-shi, Saitama-ken, 350-02, Japan.
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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

Callus induction starting from the rhizome tissue of plants of three Curcuma species: Curcuma zedoaria Roscoe, C. domestica Valeton, and C. aromatica Salisbury (Zingiberaceae) was performed. Successful callus formation was obtained in all three species using Murashige-Skoog's (MS) medium supplemented with 1-naphthalene-acetic acid (NAA) (1 ppm) and kinetin (0.1 ppm). Plantlet formation of C. zedoaria from stem tips was successfully conducted on MS medium containing NAA (0-1 ppm) or 6-benzyladenine (BA)(0-3 ppm) at 26 °C under the condition of 12 h photoperiod. Production of essential oil in tissue-cultured plants of C. zedoaria were examined using gas chromatography and the same gas chromatogram patterns of essential oil as in the mother plants were detected.