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Planta Med 1988; 54(1): 82-83
DOI: 10.1055/s-2006-962346
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© Georg Thieme Verlag Stuttgart · New York

In vitro Propagation of Valeriana wallichii

J. Mathur1 , P. S. Ahuja1 , A. Mathur1 , A. K. Kukreja1 , N. C. Shah2
  • 1Division of Plant Tissue Culture, Central Institute of Medicinal and Aromatic Plants (CIMAP), P.B. No. 1, R. S. M. Nagar, Lucknow-226016, India.
  • 2Division of Botany and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants (CIMAP), P.B. No. 1, R.S.M. Nagar, Lucknow-226016, India.
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Publication History

1987

Publication Date:
24 January 2007 (online)

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Abstract

A tissue culture procedure has been developed for the rapid multiplication of Valeriana wallichii D C. through shoot tip and axillary bud explants. MS medium containing Kn or BAP (5.0 mg/l-1) in combination with IAA (1.0 mg/l-1) induced an optimal growth of shoots within 6-8 days from both apical and axillary bud explants. The roots developed on the same medium within 2-3 weeks. Hardening of in vitro grown plantlets in pots under glass-house conditions was dependent upon the temperature and humidity. A cold-temperate climate favoured early establishment. Following the given procedure, a large number of plants have been established under field conditions at two locations. The method has implications in the early introduction of an elite population as well as its improvement.