Somatic Embryogenesis and Clonal Multiplication of Panax ginseng

Abstract

Embryogenesis of Panax ginseng was induced from young flower buds via callus during a 3 months culture period. Matured embryos could be germinated on the 1/2 Murashige and Skoog's medium supplemented with GA₃ (1.4 µM)-BAP (2.2 µM) and 1.5% sucrose. In the medium containing 1.4 µM GA₃ and 11.1 µM BAP, a multiple shoot complex having 8 shoots per segment was formed from a single shoot set. On the other hand, the addition of 11.1 µM BAP and 1.4 µM GA₃ to the medium stimulated the flower bud formation. For root formation of shoots, the MS medium supplemented with 5.4 µM NAA was the most favourable. Subsequently the plantlet was transferred to vermiculite.