Production of the Alkaloids Emetine and Cephaeline in Callus Cultures of Cephaelis ipecacuanha

Abstract

Callus cultures were established from hypocotyl explants of Cephaelis ipecacuanha, emetine and cephaeline were identified by HPLC in callus cultures. The effect of macrosalts of different basal medium and hormones on growth and alkaloid accumulation in callus tissues was investigated. Best growth in terms of wet/dry weight was obtained on media containing macrosalts of B₅ supplemented with 3% sucrose, 8 mg l^-1 IBA, 4 mg l^-1 IAA, and 4 mg l^-1 NAA. The highest yields of alkaloids were detected in medium containing macrosalts of SH with same hormone combination.