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Planta Med 1987; 53(1): 87-90
DOI: 10.1055/s-2006-962629
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© Georg Thieme Verlag Stuttgart · New York

A Competitive Solid-Phase Enzyme Immunoassay for the Evaluation of Tropane Alkaloids in Plant Material, using Anti-DL-Tropic Acid Antibodies

M. A. Fliniaux, A. Jacquin-Dubreuil
  • Department of Pharmacognosy, U.F.R. of Pharmacy, 3, Place Louis Dewailly, F-80037, Amiens Cédex, France.
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Publication History

1986

Publication Date:
24 January 2007 (online)

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Abstract

Antibodies were obtained from the immunization of rabbits with DL-tropic acid conjugated to bovine serum albumin. Their wide specificity enabled us to analyse simultaneously every main tropane alkaloid of Solanaceous plants: hyoscyamine, atropine (DL-hyoscyamine), and scopolamine. The competitive ELISA assay used for this analysis was found to be a very rapid, sensitive method with good accuracy for the dosage of alkaloids in plant material: purified extracts, crude extracts, and dry powdered material. A tropine could be detected with a sensitivity of 0.1 ng. A racemization step was necessary to detect hyoscyamine and scopolamine with the same sensitivity.