Assay Method for Antihepatotoxic Activity Using Complement-Mediated Cytotoxicity in Primary Cultured Hepatocytes1

Yoshinobu Kiso; Yutaka Kawakami; Kumiko Kikuchi; Hiroshi Hikino

doi:10.1055/s-2006-962692

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Planta Med 1987; 53(3): 241-247
DOI: 10.1055/s-2006-962692

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Assay Method for Antihepatotoxic Activity Using Complement-Mediated Cytotoxicity in Primary Cultured Hepatocytes¹

Yoshinobu Kiso, Yutaka Kawakami, Kumiko Kikuchi, Hiroshi Hikino

Pharmaceutical Institute, Tohoku University, Aoba-yama, Sendai 980, Japan.

1 Liver-protective drugs, Part 35. Also Part 120 in the series on the validity of the Oriental medicines.

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Publication History

1986

Publication Date:
24 January 2007 (online)

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Abstract

Conditions were investigated to devise an in vitro assay method for antihepatotoxic activity using complement-mediated cytotoxicity in primary cultured mouse liver cells. Utilizing 3 h preincubated hepatocytes, anti-serum against liver specific lipoprotein and guinea pig complement, a satisfactory assay procedure was achieved. Some natural products known to exert liver-protective effects in experimental liver damages were subjected to screening by this assay method to reveal that cynarin, glycyrrhizin, picroside II, glycyrrhetinic acid, silybin, and picroside I exhibited significant antihepatotoxic activity but no remarkable prevention was observed in desoxypodophyllotoxin which markedly inhibited carbon tetrachloride-, D-galactosamine-, and peroxides-induced Cytotoxicity in primary cultured hepatocytes. The utility of this assay method is discussed.