Planta Medica, Table of Contents Planta Med 1987; 53(6): 521-525DOI: 10.1055/s-2006-962800 Full Papers © Georg Thieme Verlag Stuttgart · New York Effects of an Active Substance Isolated from the Roots of Angelica shkiokiana on Leukotriene and Monohydroxyeicosatetreaenoic Acid Biosyntheses in Human Polymorphonuclear Leukocytes Yoshiyuki Kimura1 , Hiromichi Okuda1 , Kimiye Baba2 , Mitsugi Kozawa2 , Shigeru Arichi3 12nd Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-02, Japan. 2Osaka University of Pharmaceutical Sciences, Kawai 2-10-65, Matsubara, Osaka 580, Japan. 3The Research Institute of Oriental Medicine, Kinki University, Sayama-cho, Minamikawachi-gun, Osaka 589, Japan. Recommend Article Abstract PDF Download Buy Article Abstract It was found that the EtOAc fraction of the roots of Angelica shkiokiana Makino inhibited calcium ionophore-induced LTB4 and LTC4 formations in human polymorphonuclear leukocytes (PMN-L). And the active substance isolated from this root of was elucidated to be 3′ (R), 4′ (R)-3′-epoxyangeloyloxy-4′-acetoxy-3′, 4′-dihydroseselin (YN-1). Furthermore, YN-I at concentrations of 4-400 µg/ml inhibited calcium ionophore-induced 12-HETE, 5-HETE, LTB4, and LTC4 formation in human PMN-L, dose-dependently, as shown by measurements with reversed phase HPLC and radioimmunoassay systems. PDF (409 kb)
