Horm Metab Res 1991; 23(3): 113-116
DOI: 10.1055/s-2007-1003628
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© Georg Thieme Verlag, Stuttgart · New York

Effects of 1,25-Dihydroxy-Vitamin D3 on Phosphate Accumulation by Myoblasts

B. Teresita, B. Ricardo
  • Departamento de Biología, Universidad Nacional del Sur, Bahía Blanca, Argentina
Further Information

Publication History

1989

1990

Publication Date:
14 March 2008 (online)

Summary

The effects of 1,25-dihydroxy-Vitamin D3 on phosphate uptake by cultured chick embryonic muscle cells were investigated. Preincubation of primary myoblast cultures during 4-24 hours with physiological levels of 1,25(OH)2D3 resulted in a significant stimulation of velocity and total capacity of phosphate accumulation by the cells. Maximal responses were obtained at 8 hours of treatment with the sterol. In agreement with previous studies, 25-hydroxy-Vitamin D3 also stimulated myoblast phosphate uptake. 24,25-dihydroxy-Vitamin D3 and vitamin D3 were ineffective. Evidence was obtained indicating that 1,25(OH)2D3 affects the Na+-linked component of muscle cell phosphate uptake through a mechanism dependent on “de movo” protein and RNA synthesis