Summary
Isoelectric focusing studies indicated that three peaks of DNA polymerase β (β-enzyme)
activity were focused at isoelectric point of 7.1, 8.2 and 9.1 in testes of sham-operated
rats, whereas in testes of hypophysectomized rats only two peaks of the β-enzyme activity
appeared at pH 7.1 and 9.1 and the peak at pH 8.2 was reconstituted by the treatment
of gonadotropins and testosterone. In the brain hypophysectomy did not affect the
activities of isoelectrically different β-enzymes.
These facts imply that under in vivo conditions the β-enzymes in testes focused at different isoelectric points are modified
with unknown factors and the β-enzyme focused at pH 8.2 is affected by pituitary hormones.
Key words
Electrofocusing - DNA Polymerase - Hypophysectomized Rat Testis
