Effect of Triiodothyronine and Thyroxine on Mitochondrial α-Glycerophosphate Dehydrogenase Activity and Mitochondrial Protein Content of Liver, Muscle and Brain of Toad, Bufo melanostictus

Summary

The responsiveness of the adult toad to triiodothyronine (T₃) and thyroxine (T₄) was studied by measuring the mitochondrial α-glycerophosphate dehydrogenase activity and mitochondrial protein content of liver, muscle and brain of toad. Both T₃ and T₄ increased the α-GPD activity and mitochondrial protein content of liver and muscle of toad. The extent of increase in the α-GPD activity and mitochondrial protein content were more pronounced with T₃ than with T₄. Further that the muscle exhibited more α-GPD activity than liver, whenever liver showed greater mitochondrial protein content than that of muscle. Brain showed no significant change in the α-GPD activity and mitochondrial protein content. Injections of cycloheximide showed inhibition of T₃ induced changes in liver and muscle. Injection of propylthiouracil also counteracted the T₄ induced effects of liver and muscle.

Key words

α-GPD - Mitochondrial Protein - Liver - Muscle - Brain - Thyroid Hormone