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J Reconstr Microsurg 1995; 11(3): 231-235
DOI: 10.1055/s-2007-1006537
ORIGINAL ARTICLE

© 1995 by Thieme Medical Publishers, Inc.

Characterization of Neurotrophic Activity in the Silicone-Chamber Model for Nerve Regeneration

Nils Danielsen, Silvio Varon
  • Departments of Experimental Research and Hand Surgery, Malmö University Hospital, University of Lund, Malmö, Sweden, and Department of Biology, University of California, San Diego, La Jolla, California
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Publication History

Accepted for publication 1994

Publication Date:
08 March 2008 (online)

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ABSTRACT

In the present study, the authors reevaluated the temporal course and properties of neurotrophic activities present in the fluid accumulating in the silicone-chamber model for nerve regeneration. The fluid collected from silicone chambers was tested in four different dissociated neuronal cell cultures. Furthermore, the activity of the chamber fluid was examined, using a cell blot technique. There was one major peak of neurotrophic activity and this activity peaked early, about 3 to 6 hr after nerve injury. Results also indicate that the chamber fluid contains at least two types of neurotrophic activities, namely nerve growth factor and ciliary neurotrophic factor.