We have developed a radioimmunoassay (RIA) for nonenzymatically glycated serum proteins.
The polyclonal antibodies prepared against reduced glycated human albumin were specific
for the glucitollysine residues of serum proteins. Serum proteins from diabetic patients
(n = 25) contained 5.3 ± 2.8 nmoles of glucitollysine/mg protein, compared to 2.0
± 0.2 in controls (n = 20). The intra- and inter-assay variables were 3.2-6.2% and
4.4-8.6%, respectively. Results from this assay procedure correlated well with those
from the boronate affinity chromatography procedure (r = 0.94; P < 0.001). The data
suggested that diabetic serum proteins contained at least 2.5 times as much immunochemically
detectable glucitollysine residures as normal serum proteins after reduction of the
proteins with sodium borohydride.
RIA - Nonenzymatic Glycation - Serum Proteins - Glucitollysine - Diabetes mellitus
