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Horm Metab Res 1989; 21(6): 324-327
DOI: 10.1055/s-2007-1009226
Clinical

© Georg Thieme Verlag, Stuttgart · New York

Bioactivity of Human Growth Hormone in Urine from Acromegalic Patients

T. Tanaka1 , S. Umezawa1 , H. Yano1 , I. Hibi1 , Y. Shishiba2 , A. Teramoto3 , E. Ishikawa4 , Y. Murakami5
  • 1Endocrine Research Laboratory, National Children's Medical Research Center
  • 2Department of Endocrinology and Metabolism, Toranomon Hospital
  • 3Department of Neurosurgery, Police Hospital
  • 4Department of Biochemistry, Miyazaki Medical College
  • 5Sumitomo Pharmaceutical Co, Ltd., Tokyo, Japan
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Publication History

1988

1988

Publication Date:
14 March 2008 (online)

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Summary

Bioactivity of hGH in urine from five acromegalic patients was determined by Nb2 bioassay and IM-9 receptor modulation assay (RMA). Urine samples were concentrated by immunoadsorbant chromatography, dialysis and lyophilization. The ratio of the bioactivity by Im-9 RMA and the immunoactivity by RIA was between 0.81 and 1.24(1.01 ± 0.19, mean ± SD). The ratio of the bioactivity by Nb2 bioassay and the immunoactivity ranged from 0.45 to 0.60 (1.37 ± 0.85).

Gel chromatography of hGH in urine samples measured by sensitive sandwich enzyme immunoassay showed that more than 97% of hGH activity was the 22K form.

Urinary hGH from acromegalic patients was bioactive in Nb2 bioassay and IM-9 RMA and the bioactivity showed a close correlation with the immunoactivity. The major immunoactive form of hGH (22K) seems to correspond to the bioactivity.

Key words

Bioactivity - Urinary Growth Hormone - Heterogeneity - Acromegaly