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Horm Metab Res 1988; 20(1): 28-31
DOI: 10.1055/s-2007-1010741
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Effect of 25-Hydroxy-Cholesterol on the Uptake of Corticosterone in Isolated Rat Liver Cells

J. G. M. Huijmans, H. J. Degenhart, D. J. Kortleve
  • Department of Pediatrics, Erasmus University Rotterdam, University Hospital Rotterdam, Sophia Children's Hospital, Rotterdam, The Netherlands
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Publikationsverlauf

1986

1987

Publikationsdatum:
14. März 2008 (online)

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Summary

Isolated rat liver cells are able to take up corticosterone. The Arrhenjus plot of the uptake shows a biphasic course with a change in the slope around 25°C.

25-OH-cholesterol is also taken up; this phenomenon reaches a maximum at 10-15 minutes. After preincubating liver cells at 37°C in the presence of this sterol the phase transition is shifted to a higher temperature (32°C). as shown in the Arrhenius plot of the corticosterone uptake. At the same time the uptake of corticosterone is diminished. This cannot readily be explained by direct competition. The mechanism might involve an inhibition of an active uptake mechanism caused by a change in the plasma membrane.

Key-Words

Hydroxylated Sterol - Steroids - Cell Membrane - Steroid Uptake