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Horm Metab Res 1988; 20(2): 82-85
DOI: 10.1055/s-2007-1010758
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Cholesterol Ester Hydrolysis in Isolated Rat Liver Cells. Effect of Estradiol, Dibutyryl Cyclic AMP and Free Fatty Acids

M. B. Ruiz, B. Ochoa, J. M. Gandarias, M. Lacort
  • Department of Physiology and Biochemistry, Medicine School, University of the Basque Country, Bilbao, Spain
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Publication History

1986

1987

Publication Date:
14 March 2008 (online)

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Summary

The basal activity of neutral cholesterol esterase (CEase) in isolated hepatocytes from fed rats showed a characteristic variation during incubation with an initial rise at 30 min and subsequent significant decrease at 2 h. Addition of estradiol (10-4 M) not only prevented this decrease but also caused an early increase in the activity of the enzyme (125% above basal value) after 60 min of incubation. The simultaneous addition of dibutyryl cyclic AMP (db-cAMP) (10-5 M) blocked the induction, whereas the cyclic nucleotide alone inhibited significantly the esterase activity.

When hepatocyte suspensions were incubated in the presence of different fatty acids (oleate, myristate and laurate, initial concentration 1 mM) for a period of 1 h, a reduction in the CEase activity was observed.

The present results suggest that the activity of rat liver neutral CEase may be under both direct and indirect hormonal control.

Key-Words

Cholesterol Esterase - 17-β-Estradiol - Dibutyryl Cyclic AMP - Fatty Acids - Isolated Rat Hepatocytes