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Horm Metab Res 1988; 20(2): 91-95
DOI: 10.1055/s-2007-1010760
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Multihormonal Regulation of Thyroglobulin Production by the OVNIS 6H Thyroid Cell Line

A. Aouani, S. Hovsépian, G. Fayet
  • Laboratoire de Biochimie Médicale et U 38 INSERM, Faculté de Médecine, Marseille, France
Further Information

Publication History

1986

1987

Publication Date:
14 March 2008 (online)

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Summary

The hormonal regulation of thyroglobulin production has been studied using a clone of the ovine thyroid cell line: OVNIS 6H. 3 among the 6 hormones proposed for serum replacement are required for an optimal thyroglobulin production; insulin, hydrocortisone and thyrotropin. Insulin alone stimulates thyroglobulin production. The presence of insulin is also required to observe hydrocortisone and TSH stimulations. Newborn calf serum inhibits thyroglobulin production. The best conditions for optimal thyroglobulin expression and TSH responsiveness are obtained in serumfree medium supplemented with 5 μg/ml insulin, 100 nM hydrocortisone and 1 mU/ml TSH.

Key-Words

Thyroid - Thyroglobulin - Thyrotropin - Insulin - Hydrocortisone