Efficacy of Glucose, Ouabain and an Aldose Reductase Inhibitor on 2-[3H] Myo-Inositol Uptake by Human, Rat and Rabbit Erythrocytes

N. Hotta; H. Kakuta; H. Fukasawa; M. Kimura; N. Koh; H. Matsumae; M. Fujimaki; N. Sakamoto

doi:10.1055/s-2007-1010843

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1988; 20(7): 387-390
DOI: 10.1055/s-2007-1010843

ORIGINALS

Efficacy of Glucose, Ouabain and an Aldose Reductase Inhibitor on 2-[³H] Myo-Inositol Uptake by Human, Rat and Rabbit Erythrocytes

N. Hotta¹ , H. Kakuta¹ , H. Fukasawa¹ , M. Kimura¹ , N. Koh¹ , H. Matsumae¹ , M. Fujimaki² , N. Sakamoto¹

¹The Third Department of Internal Medicine, Nagoya University School of Medicine, Tokyo, Japan
²Daiichi Pharmaceutical Co., Ltd. Research Institute, Tokyo, Japan

Abstract

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Summary

Myo-inositol uptake by erythrocytes from humans, rabbits and rats was studied with an isotope technique. In human erythrocytes, the inhibitory effect on myo-inositol uptake was stronger with glucose than with ouabain. However, an aldose reductase inhibitor (ONO-2235, 100 μM) or insulin (200 μU/ml) failed to correct the decrease in myo-inositol uptake in packed RBC, produced by either 10 mM glucose or 2 mM ouabain. Ten mM ouabain had an inhibitory effect on myo-inositol uptake in all species, but an inhibitory effect was not observed with 20 mM glucose in rabbit erythrocytes. The results suggest that myo-inositol uptake by erythrocytes may be dependent on the active transport system via sodium-ATPase and that erythrocytes may not be a suitable model to monitor the possible effect of an aldose reductase inhibitor on myo-inositol concentrations in other tissues concerned with diabetic complications.

Key-Words

Myo-Inositol Glucose - Ouabain - Aldose Reductase Inhibitor - ONO-2235

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