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DOI: 10.1055/s-2007-1010906
© Georg Thieme Verlag, Stuttgart · New York
The Effect of High Glucose and High Insulin Concentrations on Pentose Phosphate Shunt Enzymes and Malic Enzyme in Cultured Human Endothelial Cells
Publication History
1987
1987
Publication Date:
14 March 2008 (online)
![](https://www.thieme-connect.de/media/hmr/198810/lookinside/thumbnails/10.1055-s-2007-1010906-1.jpg)
Summary
The influence of glucose and insulin on pentose phosphate shunt enzymes and malic enzyme activity in cultured human endothelial cells has been investigated. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malic enzyme were present in endothelial cells. Enzyme activities were not altered either by 20 mM glucose or 10-8 M insulin after 3, 6 and 12 hour incubations respectively.
Neither increased glucose nor increased insulin alter the activity of the pentose phosphate shunt. As a consequence fatty acid and cholesterol synthesis in the endothelial cell is unlikely to be altered in the presence of increased glucose or increased insulin.
Key-Words
Endothelial Cell - Glucose - Insulin - Pentose Phosphate Shunt