Atrial Natriuretic Peptide Concentration and Natriuretic Hormone Activity in Plasma of Patients with Chronic Renal Failure

Summary

To elucidate further the possible role of atrial natriuretic peptide (ANP) and hypothetical natriuretic hormone (NH) in volume and BP regulation in chronic renal failure (CRF) we measured plasma ANP, digitalis-like substances (DLS) and Na⁺-K⁺-ATPase activity (using ⁸⁶Rb influx into RBC) in 9 patients with CRF before and after hemodialysis. Volume expansion between consecutive dialyses led in all patients to the elevation of plasma ANP (83.4±14.2 pmol/l) reaching in some overhydrated subjects and/or patients with concomitant cardiac insufficiency concentration > 150 pmol/l. Reduced ⁸⁶Rb influx into RBC before hemodialysis (37.7±4.9% of controls) was accompanied by higher DLS concentrations (201±32 pmol/l). Ultrafiltration during hemodialysis with ECFV reduction lowered both ANP and DLS concentrations to 28.1±9.4 pmol/l and to 151±23 pmol/l, respectively, and abolished partly the inhibition of Na⁺-K⁺-ATPase activity (64.9±7.6% of controls). These changes corresponded to the degree of ECFV alteration. Our results suggest that both natriuretic principles are activated during ECFV expansion in CRF, probably as a corrective mechanism, with a tendency to normalize when ECFV is reduced during hemodialysis.

Key-Words

Atrial Natriuretic Peptide - Endogenous Digitalis-Like Substance - Na⁺-K⁺-ATPase - Chronic Renal Failure - Hemodialysis