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Horm Metab Res 1987; 19(2): 62-64
DOI: 10.1055/s-2007-1011739
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Spent Media from Immature Seminiferous Tubules and Sertoli Cells Inhibit Adult Rat Leydig Cell Aromatase Activity

V. Papadopoulos, P. Kamtchouing, M. A. Drosdowsky, S. Carreau
  • Laboratoire de Biochimie, CHU Côte de Nacre, Caen, France
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Publikationsverlauf

1985

1986

Publikationsdatum:
14. März 2008 (online)

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Summary

Adult rat Leydig cell aromatase activity is stimulated 2.5fold by LH or dbcAMP. Spent media prepared from seminiferous tubules or Sertoli cells of immature rats depress both the basal and the LH stimulated estradiol syntheses (25 and 20% decreases, respectively). These inhibitory effects are further enhanced when FSH is added to the culture medium of seminiferous tubules or Sertoli cells. Rat serum as well as culture media from other cell lines are ineffective while seminiferous tubule media from other immature animals (mouse, guinea-pig, calf) inhibit the aromatase activity. This Sertoli cell factor is a heat stable protein (molecular weight > 10kDa), different from the LHRH-like Sertoli cell compound, which acts on the aromatase activity at a step beyond the adenylate cyclase.

Key Words

Seminiferous Tubules - Sertoli Cells - Leydig Cell Aromatase