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Horm Metab Res 1987; 19(7): 316-318
DOI: 10.1055/s-2007-1011809
Clinical

© Georg Thieme Verlag, Stuttgart · New York

The Effect of High-Glucose Condition on Insulin Binding to IM-9 Lymphocytes

N. Watanabe, M. Kobayashi, M. Iwasaki, O. Ishibashi, Y. Takata, Y. Shigeta
  • The Third Department of Medicine, Shiga University of Medical Science, Ohtsu, Shiga, Japan
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Publication History

1986

1986

Publication Date:
14 March 2008 (online)

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Summary

The long-term effect of high-glucose condition on insulin binding to IM-9 cells was studied by incubating the cells in RPMI-1640 medium containing 450 mg/dl of glucose.

Insulin binding began to decrease after incubation for 6 days in high-glucose-treated cells, and significantly decreased after 14 days due to the reduction of receptor affinity.

The number of binding sites for insulin did not change by the treatment.

Thus, high-glucose condition per se does not appear to induce the decrease of the number of insulin receptors on target cells, as observed in diabetic patients.

Key-Words

Insulin Binding - High Glucose Condition - Lymphocytes