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Horm Metab Res 1987; 19(11): 563-564
DOI: 10.1055/s-2007-1011882
ORIGINALS
Basic
© Georg Thieme Verlag, Stuttgart · New York

Action of Calcitonin Gene-Related Peptide at the Calcitonin Receptor of the T47D Cell Line

F. Raue, H. G. Schneider, A. Zink, R. Ziegler
  • Abteilung Innere Med. I - Endokrinologie und Stoffwechsel, Universität Heidelberg, Heidelberg, Germany
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Publication History

Publication Date:
14 March 2008 (online)

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Summary

Some effects of calcitonin (CT) can also be produced by calcitonin gene-related peptide (CGRP), an alternative product of the calcitonin gene. This might be mediated by interaction of CGRP at the CT-receptor site. The human breast cancer cell line T47D possesses well characterized CT-receptors (KD = 2.3 × 10-10M for 125I salmon CT). 50% inhibition of 125I-sCT binding was achieved with 10-5 M sCT, 5 × 10-6M rat CGRP and 10-5M human CGRP. Half maximal cAMP production in T47D cells was seen with 6 × 10-10 M sCT, 5 × 10-6M rCGRP and 10-5M hCGRP. Binding and displacement capacity as well as the biological activity of CT and CGRP seems to correlate well. These findings suggest that CGRP in pharmacological doses acts via the CT-receptor. This could be explained by the homology and conformational similarities between CT and CGRP.

Key-Words

Calcitonin Receptor - Calcitonin Gene-Related Peptide - T47D Cell Line - cAMP