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Horm Metab Res 1986; 18(4): 221-224
DOI: 10.1055/s-2007-1012278
ORIGINALS
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© Georg Thieme Verlag, Stuttgart · New York

Effect of Temperature upon Potassium-Stimulated Insulin Release and Calcium Entry in Mouse and Rat Islets

C. M. Dawson, P. Lebrun1 , A. Herchuelz1 , W. J. Malaisse1 , A. A. Gonçalves, I. Atwater
  • Department of Biophysics, School of Biological Sciences, University of East Anglia, Norwich, United Kingdom
  • 1Laboratories of Experimental Medicine and Pharmacology, Brussels University School of Medicine, Brussels, Belgium
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Publication History

1984

1985

Publication Date:
14 March 2008 (online)

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Summary

The effect of cooling to 27° C was studied in islets of Langerhans exposed to 5 and 50 mM potassium in the absence of glucose. Membrane potential and insulin release were measured simultaneously from microdissected mouse islets while 45Ca outflow and insulin release were measured from collagenase-isolated rat islets. Cooling inhibited potassium-induced insulin release in both preparations. However, calcium entry estimated from electrical records and from 45Ca outflow experiments was only slightly affected by decreasing the temperature to 27° C. It is concluded that the inhibition of insulin release caused by cooling to 27° C can, within limits, be dissociated from calium influx.

Key-Words

Insulin Release - Islets of Langerhans - Calcium Entry - Temperature Effects