Summary
Stable glycosylated haemoglobins and glycosylated plasma proteins were determined
by affinity chromatography using Glycogel B in animal models with diabetes or inappropriate
hypoglycaemia. Adult Aston ob/ob mice and C57BL/KsJ db/db mice exhibited 1.5-1.9 fold increases of body weight, 2.5-3.4 fold elevations of
plasma glucose and 20.9-29.3 fold elevations of plasma insulin. Glycosylated haemoglobins
and glycosylated plasma proteins were raised 7.2-8.2 fold and 6.6-6.7 fold respectively.
In adult NEDH rats, administration of streptozotocin or implantation of transplantable
insulinoma fragments produced reciprocal changes in insulin and glucose concentrations
either resulting in the onset of insulin deficiency (5.9 fold decrease) and hyperglycaemia
(3.2 fold increase) by 2 days, or hyperinsulinaemia (2.1 fold increase) and hypoglycaemia
(1.4 fold decrease) by 6 and 8 days, respectively. Glycosylated plasma proteins were
increased (1.2 fold) rapidly after streptozotocin treatment followed by glycosylated
haemoglobins (1.6 fold increase) after 8 days. In contrast, the decreases in glycosylated
plasma proteins and glycosylated haemoglobins (4.4 fold and 1.4 fold, respectively)
in insulinoma-bearing rats preceded the demonstration of hypoglycaemia by 5 and 2
days, respectively. Glycosylated plasma proteins in insulinoma-bearing rats returned
to pretransplantation values at 10-16 days. Good correlations were observed in mice
and rats between glucose and both glycosylated haemoglobins (r = 0.92 and r = 0.90,
respectively) and glycosylated plasma proteins (r = 0.85 and r = 0.93), and between
the glycosylated blood proteins themselves (r = 0.95 and r = 0.91). The results show
that the measurement of glycosylated blood proteins by affinity chromatography using
Glycogel B provides a sensitive and reliable indicator of the recent glycaemic environment.
Key-Words
Glycosylated Haemoglobins
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Glycosylated Plasma Proteins
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ob/ob Mice
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db/db Mice
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Streptozotocin Diabetic Rats
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Insulinoma Bearing Rats
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Hypoglycaemia
