Glucagon-Degrading Activity in Acid-Ethanol Extract of Rat Submandibular Gland

Y. Tahara; K. Shima; M. Hirota; A. Tanaka; H. Ikegami; Y. Yamamoto; M. Fukuda; Y. Kumahara

doi:10.1055/s-2007-1012443

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1986; 18(12): 800-803
DOI: 10.1055/s-2007-1012443

ORIGINALS

Basic
© Georg Thieme Verlag, Stuttgart · New York

Glucagon-Degrading Activity in Acid-Ethanol Extract of Rat Submandibular Gland

Y. Tahara, K. Shima¹ , M. Hirota¹ , A. Tanaka, H. Ikegami, Y. Yamamoto, M. Fukuda, Y. Kumahara

Department of Medicine and Geriatrics, Osaka University Medical School, Osaka, Japan
¹Department of Laboratory Medicine, Tokushima University School of Medicine, Tokushima, Japan

Abstract

PDF Download

Summary

To investigate whether immunoreactive glucagon really exists in salivary gland, the integrity of glucagon radio-immunoassay was tested in the acid-ethanol extract of rat submandibular gland. Though immunoreactive glucagon was apparently measured in acid-ethanol extract of rat submandibular gland, the extract contained a significant amount of intact glucagon-degrading activity. The apparent % bound in radioimmunoassay highly correlated with the degradation of [¹²⁵I] glucagon during incubation. Gel filtration profiles of [¹²⁵I] glucagon incubated with acid-ethanol extract were the same as those of [¹²⁵I] glucagon damaged by submandibular acid-saline extract. These data suggest that the immunoreactive glucagon in acid-ethanol extract is, as in the case of acid-saline extract, an artifact due to degradation of [¹²⁵I] glucagon during radioimmunoassay.

Key-Words

Immunoreactive Glucagon - Salivary Gland - Glucagon Radioimmunoassay - Acid-Ethanol Extraction - Glucagon-Degrading Activity

PDF (348 kb)