Summary
The mechanisms of islet allograft rejection are still unknown. 350 allogeneic rat
islets (BDE/Han) were transplanted into the liver of Streptozotocin-diabetic rats
(Lewis/Han). Animals were killed either on day 4, 7, 10 or 14.
Immunfluorescence analysis for islet-cell-surface-antibodies (ICSA) was performed
on BDE-islet-single-cells yielded by Collagenase-Dispase digestion. In order to detect
cytoplasmic islet cell antibodies, immunfluorescence technique was used on BDE-pancreas
cryosections. Cytotoxicity assay was tried out using 51 Cr-release-test.
After successful intraportal transplantation, rejection took place between day 7 and
10. ICSA could be observed on day 10 and 14. Cytoplasmic antibodies were not detected
at any time. A specific cytotoxic activity in the recipient serum revealed itself
on day 10 (23.0%) and day 14 (27.7%).
Transplantation of freshly isolated allogeneic islets induced ICSA and cytotoxic antibodies.
These results indicate an involvement of humoral mechanisms in islet allograft rejection.
Key-Words:
Allogeneic Islet Transplantation
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Rats
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Islet-Cell-Surface-Antibodies
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Cytoplasmic Antibodies
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Cytotoxicity Assay
