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Horm Metab Res 1984; 16(12): 626-630
DOI: 10.1055/s-2007-1014869
Basic

© Georg Thieme Verlag, Stuttgart · New York

In Vivo and In Vitro Effects of Cholecystokinin Octapeptide on the Release of Growth Hormone in Rats

M. Matsumura, A. Yamanoi, S. Yamamoto, H. Mori, S. Saito1
  • Second Department of Internal Medicine, School of Medicine, University of Tokushima, Tokushima, Japan
  • 1First Department of Internal Medicine, School of Medicine, University of Tokushima, Tokushima, Japan
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Publikationsverlauf

1983

1983

Publikationsdatum:
14. März 2008 (online)

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Summary

The effect of Cholecystokinin Octapeptide (CCK-8) on the release of growth hormone (GH) in rats was studied in vivo and in vitro. Intravenous injection of 5 μg/100 g BW of CCK-8 resulted in significant increase in the plasma GH level after 10 and 20 min. CCK-8 at concentrations of 10-11M to 10-7M also caused dose-dependent stimulation of GH release from dispersed cells of rat anterior pituitary. On the other hand, somatostatin (SRIF) inhibited GH release from dispersed cells of rat anterior pituitary in a dose-related manner at concentrations of 10-7M to 10-9M. Release of GH from the cells was increased by addition of K+ at high concentration (50 mM) in a Ca++-dependent manner. Addition of 10-3M verapamil to the incubation medium inhibited CCK-8-induced GH release from the cells. Addition of SRIF (10-7M) to the incubation medium inhibited GH release from the cells induced by CCK-8 or high K+ (50 mM).

These results indicate that CCK-8 acts directly on the anterior pituitary cells to stimulate GH release and that calcium ion is involved in the mechanism of this effect.

Key-Words:

Cholecystokinin Octapeptide - Growth Hormone - Somatostatin - Verapamil - Pituitary Cells